Body fluids presumptive tests
- Created by: meganjayne15
- Created on: 02-05-14 21:06
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- Body Fluids - Presumptive Tests
- Blood
- 8% body weight
- erthrocytes
- red blood cells
- leucocytes
- white blood cells
- thrombocytes
- platelets
- erthrocytes
- presumptive testing
- confirmatory tests
- leucocrystal violet
- amido black
- one step ABAcard hematrace
- 8% body weight
- Semen
- visual examination
- woods lamp
- blue crime light
- presumptive test
- acid-phosphatase
- high amount of acid phosphatase present in semen
- colour change reaction
- immediate colour change indicates semen
- 2 min colour change indicates vaginal fluid
- purple
- colour change reaction
- must be done in fume cupboard
- high amount of acid phosphatase present in semen
- acid-phosphatase
- confirmatory test
- PSA/P30
- Prostate Specific Antigen (p30)
- positive result when test and control are highlighted due to antigens binding
- limitations
- only used for semen
- positive result may be obtained with male urine
- Prostate Specific Antigen (p30)
- PSA/P30
- visual examination
- Saliva
- phadebas
- 1. place phadebas paper over the area to be tested with the blue reagent side in contact with the item.
- 2. spray water onto the phadebas paper
- 3. lay glass slide on top of the paper
- 4. observe for a maximum of 40 minutes.
- 3. lay glass slide on top of the paper
- 2. spray water onto the phadebas paper
- positive is identified as a distinct area of diffuse blue colour on the non-reagent side of the paper
- 1. place phadebas paper over the area to be tested with the blue reagent side in contact with the item.
- salivary amylase
- some people do not produce amylase or produce a small amount
- phadebas
- Vaginal Fluid
- visual examination
- acid phosphatase
- could give false positive
- Urine
- visual examination
- smell
- bayer multiple reagent strips for urinalysis
- Faeces
- undigested food, bacteria
- visual examination
- smell
- urobilinogen - edelmans reagent
- Vomit
- no test
- GC used to see poisons present
- visual examination
- smell
- Blood
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