Biomolecules Cells and Microorganisms
- Created by: simmys7
- Created on: 13-11-15 12:40
Chemotaxis:
Movement of an organism in response to a chemical stimulus.
Virology
Intracellular obligate parasites
Grow in host cells ('tissue culture') and look for effect
No antibiotics, specific antiviral agents.
Mycology
Fungi-grow more slowly than bacteria. Fewer infections. Few antifungal agents.
Chemopathogens
Prokaryotes
- No membrane bound organelles
- No nucleus
Eukaryotes
- Membrane bound organelles
- Nucleus
Evolution
Process driven by mutation and selection
Inversion
Chamnges base sequence to opposite way around
Review Quiz
50% of earths oxygen is produced by microoragnisms and plants
Review Quiz
Hyperthermophiles are bacterium which can survive in hot springs
Review Quiz
Staphylococcus Aureus is the nomenclature for skin bacterium which can cause food poisoning
Review Quiz
Cynobacteria is the first bacteria which produced oxygen by photosynthesis
Review Quiz
Major groups of eukaryotic organisms are:
- Algae
- Protozoa
- Fungi
Microbial Universe
Microbes are used to treat sewage, clean up industrial waste and help with fixation of nitrogen and soil from chemical fertilisers
Carbon Cycle
Primary producers e.g. plants draw energy from sunlight, taking in CO2 and releasing oxygen.
Consumers and microoranisms use oxygen and give off CO2
Virulence Determinants
Provides quantitiative measure of pathogenicity or unliklihood of causing disease.
Capsule:Antipathogenic,used to make vaccines against bacterium.
Enxymes: Cause damage to host, for example; gas gangrene, has necrotising fascitis.
Toxins: Exotoxins is secreted by bacteria and each has a specific effect. Endotoxins are part of the cell wall and are only released when the cell dies. Lots of bateria produce endotoxin and all have the same effect- fever, shock, rash and even death e.g. meinigitis where extra endotoxin is made and released into the body.
Survival inside cells: This stops our defence from attacking the invader. Tuberculosis bacteria do this, as they have a waxy cell wall which helps protect them.
Medical Microbiology
Micro-organisms are ubiquitous (everywhere) so interact with the immediate environment. They get their nutrients from the environment then release waste into the environment. This can be beneficial e.g. Yeast, pharmaceutical uses.
Normafluora-organisms that are present but depending on the environment causes disease that can sometimes benefit you.
Oportunistic Pathogen- causes infection under certain conditions.
Bacteriology
Epidemiology
Important for surveillance.
Identify 'new' emerging or re-emerging diseases
Enable control and prevention, and to monitor effects
Control strategies: Immunisation
Water treatment
Improved living conditions
Education
Pest Control
Germ Theory
Many diseases caused by presence and actions of specific micro organisms
Koch-applied germ theory to public health and hygeine practices in order to prevent disease.
Joseph Lister-Developed practical applications of germ theory of disease with respect to sanitation in medical settings and aseptic surgical techniques partly throught use of carbolic acid as an antiseptic.
Koch's Postulate
Identify the causative agent of a particular disease.
Putting the theory to the test:
1. Suspected organism present in all cases of disease and absent from healthy animals.
2.Suspected organism should be grown in pure culture.
3. Cells from a pure culture of the suspected organism should cause disease in healthy animal.
4. Organism should be re-isolated and shown to be the same as the original.
Disease
Pathology- study of disease
Pathogenesis-Disease develops
Pathogenic Microorganisms- Ability to cause disease
Virulence Factors
Allows microorganisms to establish itself and enhance potential to cause disease
Microorganisms establish relationships with other
Commensal(ism)-Bacteria that's on your skin
Mutualism- two organisms of different species exist in relationship in which each individual benefits from the activity of the other.
Paratism- non-mutual symbiotic relationship between species, where one species, the parasite, benefits at the expense of the other, the host.
Diversity of an environemt in an ecosystem
Species richness: total number of diffrent species present
Species abundance: proportion of each species in an ecosystem
Biofilms
Biofilms are assemblages of bacteria cells adhered to a surface and unclosed in an adhesive matrix excreted by the cells. The matrix consists of a mixture of polysaccharides (chains of carbohydrates)
Biofiulms trap nutrients from microbial growth and help prevent detachment of cells in flowing systems.
Example;
Pseudomonas aeruginosa
- Produces biofilms
- Intracellular communication (quorum sensing) is critical in the maintenance of a biofilm
- Interspecies signalling (communication within species) and Intraspecies signalling
- increased resistance to antibiotics
Limitations
Biofilms have been implicated in several medical and dental conditions e.g. periodontal disease(affects soft and hard structures that support the teeth), kidney stones and others.
In industrial settings biofilms can slow the flow of liquids through pipelines and can accelerate corrosion of inert surfaces.
Human microbiome project (HMP)
Designed to understand the microbial components of our genetics and metabolic landscape which are associated with both healthy and diseased humans
Microbial growth- Yeast budding
Microbial growth
Actinomysetes-Any member of a heterogeneous group of gram-positive, generally anaerobic bacteria
Filamentous fungi- generally appearing as a circular colony that may be cottony or wooly but with filaments that are not organized into large fruiting bodies, such as mushrooms.
Batch culture
Grow in liquid medium e.g. universal bottles and flasks of liquid medium, fermenters.
Lag phase-transition phase for organisms ot ajust to the new environment. length of lag phase depends on the previous environment e.g. pH, growth medium, temperature etc. to reduce the time in lag phase create optimum conditions to increase productivity.
Exponential Phase (Log phase)- period of balanced growth, biomass increases logarithmically. Growth is characterised by increase in cell number and increase in cell size. maximum growth rate is determined by conditions e.g. carbon and energy sources, temperature, pH, water availability etc.
Deceleration Phase- rate of growth decreases due to nutrient limitation (e.g. C,N or P source) and toxicity/stress e.g. too many organisms, oxygen depletion, over production of acid/alcohol.
Stationary phase- is the decrease in primary metabolic activity, production of secondary metabolites e.g. antibiotics, decrease in cell viability, cell walls start to leak leading to death.
Key factors affecting rate of growth
- Temperature
- pH
- Water activity
- oxygen
- presence of chemical co-factors/inhibitors/toxins
Microbial growth-Factors Affecting growth
Log scale/pH scale- causes a change in hydrogen ion (H+) concentration.
Organisms can be; Acidophiles (acid tolerant) or Alkaliphiles.
Organisms Minimum pH Optimum pH Maximum pH
Thiobacillus Thiooxidans 0.5 2.0-2.8 4.0-6.0 <--Acidophiles
Lactobacillus acidophilus 4.0-4.6 5.8-6.6 6.8 <--Yogurt Production
Staphylococcus aureus 4.2 7.0-7.5 9.3<--Acid tolerant
Microbial growth-Temperature
Temperature
Psychrophile- extremophilic bacteria or archaea which are cold‐loving, having an optimal temperature for growth at about 15°C or lower, a maximal temperature for growth at about 20°C and a minimal temperature for growth at 0°C or lower
Mesophile- an organism that can grow best in moderate temperatures typically between 20-45°C (Humans)
Thermophile-a bacterium or other microorganism that grows best at higher than normal temperatures.
This can occur due to lipid content of membrane and enzymes.
Minimum°C Optimum°C Maximum °C Example
Psychrophiles <0 4 12 Polarmonas Vacuolata
Mesophiles 9 39 48 Escherichia coli
Thermophiles 42 60 68 (Bacillus) Stearothermophilus
Hyperthermophiles 65 88 98 Thermococcus celer
Microbial growth-Water Availability
Most cells = 70-90% water
Not all water is available to cells, some is;
- Physically bound (absorbed to surfaces)
- Chemically bound (associated with e.g. salts and sugars)
Toxic Products Enzyme which destroys product Superoxide (O2-) Superoxide dismutase (SOD) AnaerobesNo respiratory systemMicrobial growth-Oxygen
Hydrogen peroxide (H2O2) Catalast, peroxidose
Hydroxyl radical (OH) N/A (transient)-Highly reactive
Cannot use O2 as terminal electron acceptor
Obligate anaerobes-are killed by oxygen and do not possess enzymes
Aerotolerant anaerobes-O2 isn't reqiured but is tolerated
Problem- Removing oxygen
Aerobes
O2 is required for respiration
Obligate aerobes- Require oxygen to grow Facultative aerobes- can switch between aerobic (respiratory) and anaerobic (fermentative) metabolism
Problem-Providing sufficient oxygen
Bacteria of medical importance
Difference between prokaryotes and Eukaryotes
- Prokaryotic microorganisms- no organelles, reproduce asexually, nucleus isn't covered with a membrane.
Metabolic tests (biochemical tests)
Dilute bacteria, colour chnage shows that the bacteria is able to produce a certain enzyme.
Antibiotic sensitivity test
- Put dics on agr plate
- Antibiotics will diffuse into agar plate
- Results sent to medics who decide the amount of medicine to give each patient.
Fungi
- Eukaryotic
- Produce yeast
- Pseudohyphae/Hyphae-Compose vegitative structure found in fungi
- Aspergillus fungi-have sporophores with bristly, knoblike top
- Penicillium
- Fasarium fungi-causes a variety of diseases in plants and animals, cases loss of fingernails and often blindness in humans
Virus
- Needs a host to survive in
- Simple structure
- specific activity e.g. HIV, AIDS-destroys immune system of host e.g. staphylococcus aureus (gram positive)
Example;
Pseudomonas- Gram negative rod, which affects body organs e.g. lungs
C.albicans- can cause oral and genetical effects in humans, occurs in humans that are not immunocompramised
Epidemiology
When/Where disease occurs and how they get there, they're transmitted in populations.
Epidemiology focusses on:
- Control and prevention procedures
- immunisation
- water treatment
- pest control
- food hygeine
Virology
Viruses can be visualised by an electron micrscope however can only see the effect it has on a host via light microscope
They thrive within living host cells- Obligate. therefore will only reproduce inside host cells
They consist of either DNA or RNA surrounded by a protective coat of protein to create genetic material.
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
Virology-Protein Coat
Genome is encased in a protein shell (capsid)
Capsid gives the virus shape, Helical, complex and icosahedral
Repeating subunits- capsomeres
Capsid + Genome = nucleocapsid
Helical
Capsomers are helically wound (rod-shape)
Intimately associated with Nucleic Acid (RNA)
Rigid or flexible helices
- tabacco mosaic virus (rigid)
- Rabies (enveloped rigid)
- Influenza, measeles, mumps, ebola
Icosahedral
20 sided polygon
Equilateral triangle
12 vertices
Virology-Replication of a virus
Attachment- produce lysosomes by phage to break down bacterial ceell wall-creating damage for infection.
Penetrate-DNA, genome
Synthesis
Assemble, package-maturation
Release & Burst-Causes host cell to burst releasing virus
Application to synthesis-eclipse phase
Attachment to maturation-latent phase
Genes code for features
Virology-Bacterial Phage
Viruses that infect bacteria
Two types of bacteriophage reproduction;
- Lytic phage:
Call gets destroyed - Lysogenic phage
Cell doesnt get destroyed
Genome incorporated into host DNA (prophage)
Codes for e.g. diphtheria toxin, scarlet fever toxin
Virology-Classification
- Classify DNA+RNA by capsid symmetry
- Enveloped or naked viruses
- Number of capsomeres
- Particle size
- Nuceic acid size
All depends on capsid size
Virology-Small infectious agents
Entire infectious unit 'virion'
Viroid, infectious RNA
Transmissible spongiform encephalopathies (TSEs)-prions-proteinaceous infectious agent- a group of progressive conditions that affect the brain and nervous system of many animals including humans.
Virology-Cultivation
Put virus in host (animals, embryonated egg, tissue culture)
Host; plant - tissue culture
Host; Bacteria - Liquid media (clears due to lysis)
Solid medium (plaques-each one 'pure culture')
Virology-Assay
An assay is an investigative (analytic) procedure in labratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entitiy (the analyte)
Phage-plaques in bacteria
Plant virus-Local lesions in plants
Virology-Diagnosis
Symptoms; epidemic behaviour, circumstances (e.g. hepatitis B after transfusion)
CPE (cytopathic effect) in tissue culture; cytology, immunofluorescence
Immunological tests for viral antigens or host antibody etc. (haemagglutination, haemadsorption, neutralisation tests)
Detection of specific products (e.g. reverse transcriptase); molecular techniques
Visualisation of virus by electron microscopy
The world of prokaryotes
Three domains of life:
Bacteria
- prokaryotes, living organisms- unicellular
Archaea
Eukarya- Eukaryotes- microorganisms (uni-cellular and multi-cellular)
The world of prokaryotes-History
Discovery of microscope in early 1600s
Anthony van Leeuwenhoek-discovered bacteria
Famous scientists
- Louis Pasteur
- Robert Koch-specific microorganisms cause specific diseases, cloning
- Sergei Winogradsky
- Alexander Fleming
How do microorganisms appear to life?
Self-generating (non-living matter)
Generation from themselves
The world of prokaryotes-History
Discovery of microscope in early 1600s
Anthony van Leeuwenhoek-discovered bacteria
Famous scientists
- Louis Pasteur
- Robert Koch-specific microorganisms cause specific diseases, cloning
- Sergei Winogradsky
- Alexander Fleming
How do microorganisms appear to life?
Self-generating (non-living matter)
Generation from themselves
The world of prokaryotes-Bacteria
Bacteria are unicellular prokaryotic microorganisms
They can;
- Independently metabolise
- Grow and Reproduce
- Differentiate
- Communicate
- Evolve
Prokaryotic cells contain;
- Cytoplasm
- Nucleoid
- Ribosomes
- Plasmid
- Cell wall
- Pilli
Area to volume ratio is higher in small cells- as they exchange substances quicker, therefore a greater chance of survival
The world of prokaryotes-4 Main classes of compoun
These differ from non-living organic matter:
Proteins- structural components of the cell,enzymes and regulators; encoded by genes; synthesised in ribosomes
Nucleic acids-Genetic material (DNA and RNA, substances of heredity)
Lipids- components of cellular membranes, cell wall and storage granules
Polysaccherides-Components of cell walls and capsule
The world of prokaryotes-Classes of compounds
1. Metabolism
Uptake nutrients from the environment, their transformation within the cell, and elimination of wastes into the environment. The cell is thus an open system.
Metabolic activities make cells chemically very complex and diverse.
2.Reproduction (growth)
Chemicals from the environment are turned into new cells under the direction of pre-existing cells.
Reproduction is based on:
a) replication of DNA and
b) equal distribution of replicated DNA into two cells after division of the initial cell.
Reproduction is the fundamental feature of the life. Ensuring existence of species and their evolution.
3) Differentiation
Formation of a new cell structure such as a spore, usually as part of a cellular life cycle. Differentiation is not a compulsory property of all microbial cells,but those species that form spores survive better in unfavourable conditions.
4) Communicaiton
Cells communicate or interact primarily by means of chemicals that are released or taken up.
One of these consequences of cells communications is the quorum sensing mechanism.
5) Movement
Living organisms are often capable of self-propulsion.
Motility is not compulsory propert of all microbes
6) Evolution
Cells contain genes and evolve to display new biological properties. Phylogenetic trees show the evolutionary relationship between cells.
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