- Fructose-6-phosphate --> fructose-1,6- bisphosphate
- Enzyme = phosphofructokinase (PFK)
- Requires ATP and Mg2+
-The molecule is further activated ready for splitting
PFK is stimulated by adrenaline and fructose-1,6-bisphosphate stimulates the pyruvate kinase enzyme, so when adrenaline is released (e.g. during exercise), more fructose- 1,6- bisphosphate is produced, leading to more production of pyruvate by stimulating pyruvate kinase
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