Biology Unit 6 - DNA Profiling 0.0 / 5 ? BiologyHealth, illness and diseaseA2/A-levelEdexcel Created by: lauraking20Created on: 11-06-17 12:15 What is a DNA profile? A fingerprint of an organisms DNA 1 of 20 Where can a sample of DNA be obtained from? Saliva, blood etc 2 of 20 What is the PCR used to do? Make millions of copies of specific regions of DNA in just a few hours 3 of 20 Why does the DNA need to be amplified? So there's enough to make a DNA profile 4 of 20 What does the reaction mixture contain? (3) DNA sample, free nucleotides, primers, DNA polymerase 5 of 20 What are primers? Short pieces of DNA that are comp. to the bases at the start of the fragment you want 6 of 20 What does DNA polymerase do? An enzyme that creates the new DNA strands (using com. base pairing) 7 of 20 3 temperatures? 90, 55, 70 8 of 20 90? Break the hydrogen bonds between the 2 strands 9 of 20 55? Primers can bond to the strands 10 of 20 70? So DNA polymerase can work 11 of 20 How many copies of DNA in one cycle of PCR? 2 12 of 20 Why may a fluorescent tag be added after? So the fragments can be used under UV light 13 of 20 What is used to separate the DNA? Gel electrophoresis 14 of 20 What is the DNA placed in? Slab of gel (agrose?) and covered in a buffer solution 15 of 20 What is passed through the gel? An electric current 16 of 20 DNA is placed at the... cathode (-) 17 of 20 What fragments travel further? Short fragments 18 of 20 What can be done with profiles after? Compare them (e.g. crime, parent) 19 of 20 Why may DNA profiling be used on plants and animals? To stop inbreeding 20 of 20
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