DNA 0.0 / 5 ? CriminologyForensic ScienceUniversityOther Created by: AshleeCreated on: 04-05-15 19:00 Where can DNA be found? White blood cells, semen and saliva 1 of 18 How would you profile DNA? Polymerase chain reaction and short tandem repeats 2 of 18 What are polymerase chain reactions (PCR)? Amplifying pieces of DNA across several magnitudes, generating a thousand/million copies of a DNA sequence 3 of 18 What are short tandem repeats (STR's)? Measures the exact number of repeating nucleotides of a microsatellite 4 of 18 What is junk DNA? DNA that doesn't code for anything/make proteins 5 of 18 What is a phenotype? Inherited physical appearance from dominant alleles 6 of 18 What is a genotype? Genetic makeup of a specific characteristic due to dominant and recessive alleles 7 of 18 What is cell free DNA? Touch DNA; transferred nucleated cells; free running nucleic acids in sweat; sloughed skin cells 8 of 18 Where do you find DNA in a cell? Nucleus 9 of 18 How would you profile DNA? DNA17; YSTR's; LCN; SGM+; DNA SenCE; MtDNA 10 of 18 What is DNA17? 32 DNA components and gender indentifier; improved discrimination, sensitivity and comparability 11 of 18 What is YSTR's? Short tandem repeat on the Y chromosome 12 of 18 Why would you use YSTR's? Women have no Y chromosome; DNA of both man and women could mix during events; when dealing with sexual assaults 13 of 18 What is LCN? Low copy number 14 of 18 Why would you use LCN? Retrieve data from small or degraded samples; does 34 cycles which is more than the usual system 15 of 18 What is SGM+? Second generation multiplex plus - analyses 10 STR markers and gender indicators for 28 cycles 16 of 18 What is DNA SenCE? Enhancement from small samples; cleans and concentrates 17 of 18 What is MtDNA? Mitochondrial DNA passed down from the mother 18 of 18
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