small rna moleucles from their own promotor. they don't code for a protein.
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How many sRNA molecules does E.coli have. what percentage of the protien coding genes do they affect?
80 sRNAs, 2%
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How do sRNAs work, and what is their effect?
Base pairing with complimentary sequences within the target mRNA leading to - a. destruction, b.inhibition of translation or c. stimulation of translation
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Example - what does the sRNA oxyS bind to and what is it's effect?
it binds to flhA mRNA, which is a transcriptional activator of formate metabolism. oxyS switches off genes involved in formate metabolism
to protect genomes from mutational effects of transposable genetic elements.
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Ways scientists are using rna interference to down regulate genes
to knock down expression of a specific gene to observe the effects of its absence. to create antiviral drugs, to create novel gene therapies, to develop anti-cancer drugs.
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an example of using antisense RNA in crops.
reduction of levels of polygalacturonase in tomatoes - PG catalyses cleavage of galacturonic acid residues in cell wall pectin.
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an example of using a siRNA to prevent viral infection
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