UCL Transamination Practical

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  • Created by: cgeorge
  • Created on: 14-04-14 20:07

1. Which of these is true?

  • in this experiment, we separate amino acids on one plate using one solvent mixture and oxoacid derivatives on another plate with a different solvent mixture
  • in this experiment, we separate amino acids and oxoacids on the same plate, due to the ability of one solvent mixture to separate these simultaneously.
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Other questions in this quiz

2. (transaminases) Which amino acid has an amino group removed and where is it transferred to?

  • the alpha amino acid has an amino group removed and transferred to a 2-oxoacid
  • the beta amino acid has an amino group removed and transferred to a 2-oxoacid
  • the alpha amino acid has an amino group removed and transferred to a beta amino acid

3. Tubes 3 and 7, the reactants are pyruvate and glutamate and the products are oxoglutarate and alanine. What was the reaction?

  • no reaction
  • transamination
  • deamination

4. Tube 10, the reactants were none and none. What was the reaction?

  • transamination
  • no reaction
  • deamination

5. Tube 9 gives a standard of what?

  • the 2,4, DNPH of 2 oxo glutarate
  • the 2,4 dinitrophenyl-hydrazone of pyruvate
  • the DNPH alone

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